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lyophilised cell-free extract in a commercial mtp format  (Prozomix Inc)

 
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    Structured Review

    Prozomix Inc lyophilised cell-free extract in a commercial mtp format
    T m data from a 96‐well <t>MTP</t> made up of 92 clones of the saturation <t>library</t> <t>DERA</t> Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.
    Lyophilised Cell Free Extract In A Commercial Mtp Format, supplied by Prozomix Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mtp+format/lyophilised+cell+free+extract+in+a+commercial+mtp+format/pmc07379660-293-15-17
    Average 90 stars, based on 1 article reviews
    lyophilised cell-free extract in a commercial mtp format - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "nanoDSF as screening tool for enzyme libraries and biotechnology development"

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development

    Journal: The Febs Journal

    doi: 10.1111/febs.14696

    T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.
    Figure Legend Snippet: T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Techniques Used: Clone Assay, Standard Deviation

    Related Articles

    Clone Assay:

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development
    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).

    Standard Deviation:

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development
    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).

    Acid Assay:

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development
    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).



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    Image Search Results


    T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Journal: The Febs Journal

    Article Title: nanoDSF as screening tool for enzyme libraries and biotechnology development

    doi: 10.1111/febs.14696

    Figure Lengend Snippet: T m data from a 96‐well MTP made up of 92 clones of the saturation library DERA Lb Q54X, two wild‐type controls (wt) and two negative controls (neg) lacking the gene coding for DERA Lb and thus not giving a corresponding melting curve. The red bars correspond to the T m values ±1 standard deviation from triplicate determinations. From the entire set 32 clones were sequenced, and these bars are labelled with the amino acid found at position 54. The high reproducibility of triplicate measurements, the good agreement of identical variants and the high yield of positively determined T m data demonstrate the high sample quality and the reliability of the method.

    Article Snippet: The phylogenetically diverse 2‐deoxyribose‐5‐phosphate (DERA) library was provided as lyophilised cell‐free extract in a commercial MTP format (Prozomix Limited, Haltwhistle, UK).

    Techniques: Clone Assay, Standard Deviation